Recombinant humaan GGH-eiwit

Gamma-glutamyl hydrolase, also known as GGH, is a homodimeric protein that catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates and is a central enzyme in folyl and antifolyl poly-gamma-glutamate metabolism. GGH may play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Recombinant human GGH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Gamma-glutamyl hydrolase, GH

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.9 kDa (315aa) confirmed by MALDI-TOF

GGH, ATGP1399-10 µg, ATGP1399-20 µg, ATGP1399-50 µg, ATGP1399-100 µg, ATGP1399-250 µg, ATGP1399-500 µg, ATGP1399-1 mg, ATGP1399-10, ATGP1399-20, ATGP1399-50, ATGP1399-100, ATGP1399-250, ATGP1399-500, ATGP1399-1

Rhee M S., et al. (1998) Mol Pharmacol. 53:1040-1046.; ; Galivan J., et al. (1999) Semin Oncol. 26:33-37.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_003869.1

Q92820

Human

RPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVP VRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFD DGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTAN FHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPN AVKTAFYLAE FFVNEARKNN HHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD