Recombinant humaan GIP-eiwit

GIP, also known as glucose-dependent insulinotropic polypeptide, is an important insulin-releasing hormone of the enteroinsular axis that has a functional profile of possible therapeutic value for type 2 diabetes. This protein is an important incretin hormone released into the circulation from endocrine K-cells of the duodenum and jejunum after ingestion of food1. It was evaluated for their ability to elevate cellular cAMP production and stimulate insulin secretion. It also promotes plasma triglyceride clearance in response to oral fat loading. In liver, GIP has been shown to enhance insulin-dependent inhibition of glycogenolysis. Recombinant human GIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Gastric inhibitory polypeptide, Glucose-dependent insulinotropic polypeptide, Gastric Inhibitory Peptide

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17.3 kDa (155aa) confirmed by MALDI-TOF

GIP, Gastric inhibitory polypeptide, Glucose-dependent insulinotropic polypeptide, Gastric Inhibitory Peptide, ATGP2061-10 µg, ATGP2061-20 µg, ATGP2061-50 µg, ATGP2061-100 µg, ATGP2061-250 µg, ATGP2061-500 µg, ATGP2061-1 mg, ATGP2061-10, ATGP2061-20, ATGP2061-50, ATGP2061-100, ATGP2061-250, ATGP2061-500, ATGP2061-1

O'Harte FP. et al. (1999) Diabetes. 48:758-765; ; Gault VA. et al. (2002) Biosci Rep. 22:523-528.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol 0.1M NaCl, 2mM DTT

Signal Transduction

AAH69100

P09681

Human

MGSSHHHHHH SSGLVPRGSH MGSEKKEGHF SALPSLPVGS HAKVSSPQPR GPRYAEGTFI SDYSIAMDKI HQQDFVNWLL AQKGKKNDWK HNITQREARA LELAGQANRK EEEAVEPQSS PAKNPSDEDL LRDLLIQELL ACLLDQTNLC RLRSR