Recombinant humaan GIPC1-eiwit

GIPC1 is a PDZ domain containing protein that interacts with RGS-GAIP and the viral oncoprotein TAX, which transactivates viral and cellular promoters through interactions with various transcription factors. GIPC specifically localizes to clusters of vesicles near the plasma membrane and participates in G protein-coupled signaling pathway involved in regulating Clathrin-coated vesicular trafficking. GIPC also associates with membrane bound Semaphorin F (M-SemF), which is involved in neuronal axon growth, and it appears to regulate the subcellular distribution of M-SemF in the brain. Recombinant human GIPC1 protein, fused to at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

PDZ domain-containing protein GIPC1, C19orf3, GIPC, GLuT1CBP, Hs.6454, IIP-1, NIP, SEMCAP, SYNECTIIN, SYNECTIN, TIP-2, RGS19IP1, PDZ domain-containing protein GIPC1, Chromosome 19 open reading frame 3, GIPC, IIP 1, TIP 2, GIPC 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.2 kDa (353aa) confirmed by MALDI-TOF

GIPC1, PDZ domain-containing protein GIPC1, C19orf3, GIPC, GLuT1CBP, Hs.6454, IIP-1, NIP, SEMCAP, SYNECTIIN, SYNECTIN, TIP-2, RGS19IP1, PDZ domain-containing protein GIPC1, Chromosome 19 open reading frame 3, GIPC, IIP 1, TIP 2, GIPC 1, ATGP0613-10 µg, ATGP0613-20 µg, ATGP0613-50 µg, ATGP0613-100 µg, ATGP0613-250 µg, ATGP0613-500 µg, ATGP0613-1 mg, ATGP0613-10, ATGP0613-20, ATGP0613-50, ATGP0613-100, ATGP0613-250, ATGP0613-500, ATGP0613-1

Lee NY., et al. (2008) J Biol Chem. 283 (47) :32527-33. ; ; De Vries L., et al. (1998) Proc Natl Acad Sci u S A. 95 (21) :12340-5. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 1mM DTT, 0.1M NaCl, and 1mM EDTA

Signal Transduction

NP_974199

O14908

Human

MPLGLGRRKK APPLVENEEA EPGRGGLGVG EPGPLGGGGS GGPQMGLPPP PPALRPRLVF HTQLAHGSPT GRIEGFTNVK ELYGKIAEAF RLPTAEVMFC TLNTHKVDMD KLLGGQIGLE DFIFAHVKGQ RKEVEVFKSE DALGLTITDN GAGYAFIKRI KEGSVIDHIH LISVGDMIEA INGQSLLGCR HYEVARLLKE LPRGRTFTLK LTEPRKAFDM ISQRSAGGRP GSGPQLGTGR GTLRLRSRGP ATVEDLPSAF EEKAIEKVDD LLESYMGIRD TELAATMVEL GKDKRNPDEL AEALDERLGD FAFPDEFVFD VWGAIGDAKV GRY