Recombinant humaan GLTPD1-eiwit

GLTPD1 mediates the intracellular transfer of ceramide-1-phosphate between organelle membranes and the cell membrane. This protein is required for normal structure of the Golgi stacks. GLTPD1 can bind phosphoceramides with a variety of aliphatic chains, but has a preference for lipids with saturated C16:0 or monounsaturated C18:1 aliphatic chains, and is inefficient with phosphoceramides containing lignoceryl (C24:0) . It plays a role in the regulation of the cellular levels of ceramide-1-phosphate, and thereby contributes to the regulation of phospholipase PLA2G4A activity and the release of arachidonic acid. Recombinant human GLTPD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycolipid transfer protein domain-containing protein 1, CPTP, MGC10334, glycolipid transfer protein domain containing 1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.8 kDa (237aa) confirmed by MALDI-TOF

GLTPD1, Glycolipid transfer protein domain-containing protein 1, CPTP, MGC10334, glycolipid transfer protein domain containing 1, ATGP2830-10 µg, ATGP2830-20 µg, ATGP2830-50 µg, ATGP2830-100 µg, ATGP2830-250 µg, ATGP2830-500 µg, ATGP2830-1 mg, ATGP2830-10, ATGP2830-20, ATGP2830-50, ATGP2830-100, ATGP2830-250, ATGP2830-500, ATGP2830-1

Simanshu D.K., et al. (2013) Nature 500:463-467.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 1mM DTT

Transportation

NP_001025056

Q5TA50

Human

MDDSETG FNLKVVLVSF KQCLDEKEEV LLDPYIASWK GLVRFLNSLG TIFSFISKDV VSKLRIMERL RGGPQSEHYR SLQAMVAHEL SNRLVDLERR SHHPESGCRT VLRLHRALHW LQLFLEGLRT SPEDARTSAL CADSYNASLA AYHPWVVRRA VTVAFCTLPT REVFLEAMNV GPPEQAVQML GEALPFIQRV YNVSQKLYAE HSLLDLP