Recombinant humaan Glucon eiwit

Glucagon, also known as GCG, a hormone secreted by the pancreas, raises blood glucose levels. Its effect is opposite that of insulin, which lowers blood glucose levels. The pancreas releases glucagon when blood sugar (glucose) levels fall too low. Glucagon causes the liver to convert stored glycogen into glucose, which is released into the bloodstream. Glucagon also stimulates the release of insulin, so glucose can be taken up and used by insulin-dependent tissues. Thus, glucagon and insulin are part of a feedback system that keeps blood glucose levels at a stable level. Recombinant human GCG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glucagon, GLP1, GLP2, GRPP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

12.8 kDa (112aa) confirmed by MALDI-TOF

Glucagon, Glucagon, GLP1, GLP2, GRPP, ATGP1139-10 µg, ATGP1139-20 µg, ATGP1139-50 µg, ATGP1139-100 µg, ATGP1139-250 µg, ATGP1139-500 µg, ATGP1139-1 mg, ATGP1139-10, ATGP1139-20, ATGP1139-50, ATGP1139-100, ATGP1139-250, ATGP1139-500, ATGP1139-1

Scrocchi L A., et al. (1996) Nat Med. 2:1254-1258.; ; Rouille Y., et al. (1995) J Biol Chem. 270:26488-26496.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl

Hormones & Cytokines

NP_002045

P01275

Human

MGSSHHHHHH SSGLVPRGSH MKRHDEFERH AEGTFTSDVS SYLEGQAAKE FIAWLVKGRG RRDFPEEVAI VEELGRRHAD GSFSDEMNTI LDNLAARDFI NWLIQTKITD RK