Recombinant humaan Gluconosyltransferase 1A1/UGT1A1-eiwit

UGT1A1 also known as uDP-glucuronosyltransferase 1-1. uGT1A1 is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. It is also able to catalyze glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4-methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Recombinant human uGT1A1, fused to His-tag at N-terminus, was expressed in E. coli.

UDP-glucuronosyltransferase 1-1, uDP-glucuronosyltransferase 1-1, BILIQTL1, GNT1, HuG-BR1, uDPGT, uDPGT 1-1, uGT1, uGT1A

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

54.7 kDa (488aa)

Glucuronosyltransferase1A1, UGT1A1, uDP-glucuronosyltransferase 1-1, uDP-glucuronosyltransferase 1-1, BILIQTL1, GNT1, HuG-BR1, uDPGT, uDPGT 1-1, uGT1, uGT1A, ATGP3014-10 µg, ATGP3014-20 µg, ATGP3014-50 µg, ATGP3014-100 µg, ATGP3014-250 µg, ATGP3014-500 µg, ATGP3014-1 mg, ATGP3014-10, ATGP3014-20, ATGP3014-50, ATGP3014-100, ATGP3014-250, ATGP3014-500, ATGP3014-1

Udomuksorn W., et al. (2007) Pharmacogenet. Genomics 17:1017-1029

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Metabolism

NP_000454

P22309

Human

MGSSHHHHHH SSGLVPRGSH MGSHAGKILL IPVDGSHWLS MLGAIQQLQQ RGHEIVVLAP DASLYIRDGA FYTLKTYPVP FQREDVKESF VSLGHNVFEN DSFLQRVIKT YKKIKKDSAM LLSGCSHLLH NKELMASLAE SSFDVMLTDP FLPCSPIVAQ YLSLPTVFFL HALPCSLEFE ATQCPNPFSY VPRPLSSHSD HMTFLQRVKN MLIAFSQNFL CDVVYSPYAT LASEFLQREV TVQDLLSSAS VWLFRSDFVK DYPRPIMPNM VFVGGINCLH QNPLSQEFEA YINASGEHGI VVFSLGSMVS EIPEKKAMAI ADALGKIPQT VLWRYTGTRP SNLANNTILV KWLPQNDLLG HPMTRAFITH AGSHGVYESI CNGVPMVMMP LFGDQMDNAK RMETKGAGVT LNVLEMTSED LENALKAVIN DKSYKENIMR LSSLHKDRPV EPLDLAVFWV EFVMRHKGAP HLRPAAHDLT WYQYHSLD