Recombinant humaan Glucosylceramidase/GBA-eiwit

GBA, also known as glucosylceramidase isoform 1, is a lysosomal enzyme that presumably requires a signal peptide for transport across the membrane of the rough endoplasmic reticulum and glycosylation for transport into lysosomes. Gaucher disease is due to a deficiency in the activity of the enzyme glucocerebrosidase. Recombinant human GBA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Glucosylceramidase isoform 1, GBA1, GCB, GLUC

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 80% by SDS-PAGE

56.4 kDa (503aa)

Glucosylceramidase, GBA, Glucosylceramidase isoform 1, GBA1, GCB, GLUC, ATGP3145-10 µg, ATGP3145-20 µg, ATGP3145-50 µg, ATGP3145-100 µg, ATGP3145-250 µg, ATGP3145-500 µg, ATGP3145-1 mg, ATGP3145-10, ATGP3145-20, ATGP3145-50, ATGP3145-100, ATGP3145-250, ATGP3145-500, ATGP3145-1

Sorge JA., et al. (1987) Am J Hum Genet. 41:1016-1024.; ; Salvioli R., et al. (2000) FEBS Lett. 472:17-21.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Neuroscience

NP_000148

P04062

Human