Recombinant humaan GLUD1-eiwit

Glutamate dehydrogenase 1, mitochondrial precursor, also known as GLuD1, belongs to the Glu/Leu/Phe/Val dehydrogenases family. GLuD1 has a central role in nitrogen metabolism in plants and animals. GLuD1 is found in all organisms and catalyzes the oxidative deamination of 1-glutamate to 2-oxoglutarate. Mutations in the gene encoding GLuD1 causes hyperinsulinism-hyperammonemia syndrome (HHS), which is an inherited condition characterized by high insulin and ammonia levels in the blood. GLuD1 may also be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate. Recombinant human GLuD1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Glutamate dehydrogenase 1, mitochondrial, GDH, GDH1, GLUD

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

58.4 kDa (528aa)

GLuD1, Glutamate dehydrogenase 1 mitochondrial, Glutamate dehydrogenase 1, mitochondrial, GDH, GDH1, GLuD, ATGP2653-10 µg, ATGP2653-20 µg, ATGP2653-50 µg, ATGP2653-100 µg, ATGP2653-250 µg, ATGP2653-500 µg, ATGP2653-1 mg, ATGP2653-10, ATGP2653-20, ATGP2653-50, ATGP2653-100, ATGP2653-250, ATGP2653-500, ATGP2653-1

Mastorodemos V., et al. (2009) Biochem Cell Biol. 87:505-516.; ; Tanizawa Y., et al. (2002) Diabetes. 51:712-717.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_005262

P00367

Human

SEAVADR EDDPNFFKMV EGFFDRGASI VEDKLVEDLR TRESEEQKRN RVRGILRIIK PCNHVLSLSF PIRRDDGSWE VIEGYRAQHS QHRTPCKGGI RYSTDVSVDE VKALASLMTY KCAVVDVPFG GAKAGVKINP KNYTDNELEK ITRRFTMELA KKGFIGPGID VPAPDMSTGE REMSWIADTY ASTIGHYDIN AHACVTGKPI SQGGIHGRIS ATGRGVFHGI ENFINEASYM SILGMTPGFG DKTFVVQGFG NVGLHSMRYL HRFGAKCIAV GESDGSIWNP DGIDPKELED FKLQHGSILG FPKAKPYEGS ILEADCDILI PAASEKQLTK SNAPRVKAKI IAEGANGPTT PEADKIFLER NIMVIPDLYL NAGGVTVSYF EWLKNLNHVS YGRLTFKYER DSNYHLLMSV QESLERKFGK HGGTIPIVPT AEFQDRISGA SEKDIVHSGL AYTMERSARQ IMRTAMKYNL GLDLRTAAYV NAIEKVFKVY NEAGVTFT