Recombinant humaan Glutaminesynthetase/GLUL-eiwit

GLUL also known as Glutamine synthetase. It is a trimetallic enzyme containing two divalent cation sites and one monovalent cation site per subunit. GLUL is able to regulate intracellular concentrations of glutamate and catalyzes the synthesis of glutamine form glutamate and ammonia. It is ubiquitously expressed in the human and plays a major role for many metabolic pathways such as cell proliferation, inhibition of apoptosis, and cell signaling. Recombinant Human GLUL was expressed in E. coli and purified by using conventional chromatography techniques

Specific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by absorbance at 280 nm)

> 85% by SDS-PAGE

Specific activity is > 2.000pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

42 kDa (373aa) confirmed by MALDI-TOF

GLUL, Glutamine synthetase , GLNS, GS, PIG43, PIG59, cell proliferation-inducing protein 59, GLNA, GLNA_HUMAN, GLNS, GLULGlutamate ammonia ligase, Glutamate decarboxylaseGlutamate--ammonia ligase, glutamine synthaseGlutamine synthetase, GS, PIG 43, PIG 59, PIG43PIG59, Proliferation inducing protein 43, ATGP3404-10 µg, ATGP3404-20 µg, ATGP3404-50 µg, ATGP3404-100 µg, ATGP3404-250 µg, ATGP3404-500 µg, ATGP3404-1 mg, ATGP3404-10, ATGP3404-20, ATGP3404-50, ATGP3404-100, ATGP3404-250, ATGP3404-500, ATGP3404-1

Liaw SH., et al. (1995) Protein Sci. 4 (11) : 2358-65.; ; Vermeulen T., et al. (2008) Arch Biochem Biophys. 478 (1) : 96-102.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1mM PMSF

Enzymes & Proteases

NP_001028216.1

P15104

Human

MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN