Recombinant humaan Glutaminesynthetase/GLUL-eiwit

Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency. Recombinant GLUL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is >2,800 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

Glutamine synthase, Glutamate-ammonia ligase, GLNS, Glutamate-ammonia ligase, GS, Palmitoyltransferase GLUL

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is >2,800pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.

44.2 kDa (393aa) confirmed by MALDI-TOF

GLUL, Glutamine synthetase, GS, GLNS, ATGP3431-10 µg, ATGP3431-20 µg, ATGP3431-50 µg, ATGP3431-100 µg, ATGP3431-250 µg, ATGP3431-500 µg, ATGP3431-1 mg, ATGP3431-10, ATGP3431-20, ATGP3431-50, ATGP3431-100, ATGP3431-250, ATGP3431-500, ATGP3431-1

Vermeulen T., et al, (2008) Arch Biochem Biophys. 478:96-102; ; Liaw SH., et al. (1995) Protein Sci. 4 (11) : 2358-65.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl

Enzymes & Proteases

NP_001028216

P15104

Human

MGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR RPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN