Recombinant humaan glutaredoxin 3/GLRX3-eiwit

Glutaredoxin (GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutaredoxin-3, GRX3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, PKCq-interacting protein, Thioredoxin-like protein 2, Glutaredoxin 4, GLRX4, GRX4, PICOT, TXNL2, HUSSY-22, bA500G10.4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

39.6 kDa (355aa) confirmed by MALDI-TOF

TXNL2, TXNL 2, Thioredoxin-like2, Thioredoxin-like protein 2, Thioredoxin-like 2, Thioredoxin like 2, PKC-theta-interacting protein, PKCq-interacting protein, PKCq interacting protein, PKC-interacting cousin of thioredoxin, PKC theta interacting protein, PKC interacting cousin of thioredoxin, PICOT, HUSSY-22, GRX4, GRX3, Glutaredoxin-4, Glutaredoxin4, Glutaredoxin-3, Glutaredoxin3, Glutaredoxin 4, Glutaredoxin 3, GLRX4, GLRX3, bA500G10.4, ATGP0551-10 µg, ATGP0551-20 µg, ATGP0551-50 µg, ATGP0551-100 µg, ATGP0551-250 µg, ATGP0551-500 µg, ATGP0551-1 mg, ATGP0551-010, ATGP0551-020, ATGP0551-050, ATGP0551-100, ATGP0551-250, ATGP0551-500, ATGP0551-01M

Witte S., et al. (2000) J Biol Chem 275 (3) : 1902-9.; ; Haunhorst P., et al. (2010) Biochem Biophys Res Commun. 394 (2) :372-6;

Kamisuki S, et al. Identification of proteins that bind to the neuroprotective agent neoechinulin A. (Biosci Biotechnol Biochem. 2018) {https://pubmed.ncbi.nlm.nih.gov/29447077/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Redox Proteins

NP_006532

O76003

Human

MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN