Recombinant humaan Glutaresdoxin 2/GLRX2-eiwit

Glutaredoxin-2, as known as GRX2, multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. This protein is localized both in the mitochondria and nucleus. It has anti-apoptotic activity by preventing cardiolipin oxidation and cytochrome c release. GRX2 exhibited 36% identity with GRX1 and had a disulfide active center containing the Cys-Ser-Tyr-Cys motif. Recombinant human Glutaredoxin 2, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

GLRX2, GRX2, CGI133, Glutaredoxin (thioltransferase) 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

17 kDa (154aa) confirmed by MALDI-TOF

Glutaredoxin2, ATGP0436-10 µg, ATGP0436-20 µg, ATGP0436-50 µg, ATGP0436-100 µg, ATGP0436-250 µg, ATGP0436-500 µg, ATGP0436-1 mg, ATGP0436-10, ATGP0436-20, ATGP0436-50, ATGP0436-100, ATGP0436-250, ATGP0436-500, ATGP0436-1

Elliott SJ., et al. (2009) Biochemistry. 948 (18) :3813-5.; ; Das DK., et al. (2008) J Mol Cell Cardiol. 44 (2) :252-60;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1mM PMSF, 10% glycerol

Redox Proteins

NP_932066

Q9NS18

Human