Recombinant humaan Glutaresdoxin 5/GLRX5-eiwit

GLRX5 is small redox enzymes of approximately one hundred amino-acid residues which uses glutathione as a cofactor. This protein is oxidized by substrates, and reduced non-enzymatically by glutathione. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Recombinant human GLRX5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Glutaredoxin-related protein 5 mitochondrial, Monothiol glutaredoxin-5, Glutaredoxin 5 homolog, GLRX5, C14orf87, PR01238, GRX5

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

18.8 kDa (177aa) confirmed by MALDI-TOF

GLRX5, Glutaredoxin-related protein 5 mitochondrial, C14orf87, GRX5, Glutaredoxin-related protein 5, mitochondrial Chromosome 14 open reading frame 87, FLB4739, GLRX 5, Glutaredoxin 5 homolog, Glutaredoxin related protein 5, Glutaredoxin5, GRX5, MGC14129, PRO1238., ATGP0544-10 µg, ATGP0544-20 µg, ATGP0544-50 µg, ATGP0544-100 µg, ATGP0544-250 µg, ATGP0544-500 µg, ATGP0544-1 mg, ATGP0544-10, ATGP0544-20, ATGP0544-50, ATGP0544-100, ATGP0544-250, ATGP0544-500, ATGP0544-1

Holmgren A., et al. (1988) FEMS Microbiol. Rev. 4 (4) : 271-297.; ; Holmgren A., et al. (1988) Biochem. Soc. Trans. 16 (2) : 95-96.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Redox Proteins

NP_057501

Q86SX6

Human

MSGSLGRAAA ALLRWGRGAG GGGLWGPGVR AAGSGAGGGG SAEQLDALVK KDKVVVFLKG TPEQPQCGFS NAVVQILRLH GVRDYAAYNV LDDPELRQGI KDYSNWPTIP QVYLNGEFVG GCDILLQMHQ NGDLVEELKK LGIHSALLDE KKDQDSK