Recombinant humaan Glutathion Peroxidase 3/GPX3 (U73C) eiwit

GPX3, also known as glutathione peroxidase 3, belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. GPX3 protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. Recombinant human GPX3 (u73C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Glutathione peroxidase 3, GPx-P, GSHPx-3, GSHPx-P, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPXP, Selenoprotein GPX3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.7 kDa (227aa) confirmed by MALDI-TOF

Glutathione Peroxidase3, GPX3 (u73C), Glutathione peroxidase 3, GPx-P, GSHPx-3, GSHPx-P, ATGP1429-10 µg, ATGP1429-20 µg, ATGP1429-50 µg, ATGP1429-100 µg, ATGP1429-250 µg, ATGP1429-500 µg, ATGP1429-1 mg, ATGP1429-10, ATGP1429-20, ATGP1429-50, ATGP1429-100, ATGP1429-250, ATGP1429-500, ATGP1429-1

Esworthy R.S., et al. (1991) Arch. Biochem. Biophys. 286:330-336; ; Comhair S.A.A., et al. (2000) Am. J. Respir. Cell Mol. Biol. 23:350-354

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT, 50 mM Imidazole

Redox Proteins

NP_002075

P22352

Human

QSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNV ASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGD VNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKM DILSYMRRQA ALGVKRK