Recombinant humaan Glutathion Reductase-eiwit

Glutathione reductase, also known as GSR, is a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein and plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG) : GSSG + NADPH + H+ ->2GSH + NADP+. In most eukaryotic cells, GSR maintains the ratio of [GSH] / [GSSG], and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis. Recombinant human GSR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 45 units/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

Glutathione reductase, GLuR, GRD1

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 45unit/mg. The unit definition for glutathione reductase activity may be expressed in terms of the oxidation of NADPH or the reduction of GSSG since their molar ratio is 1:1. One unit of glutathione reductase oxidizes 1 umol of NADPH per minute at 37C, pH 7.5.

54.3 kDa (504aa) confirmed by MALDI-TOF

Glutathione Reductase, Glutathione reductase, GLuR, GRD1, ATGP1318-10 µg, ATGP1318-20 µg, ATGP1318-50 µg, ATGP1318-100 µg, ATGP1318-250 µg, ATGP1318-500 µg, ATGP1318-1 mg, ATGP1318-10, ATGP1318-20, ATGP1318-50, ATGP1318-100, ATGP1318-250, ATGP1318-500, ATGP1318-1

Stoll V S., et al. (1997) Biochemistry. 36:6437-6447.; ; Karplus P A., et al. (1987) J Mol biol. 195:701-729.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Redox Proteins

NP_000628

P00390

Human

MGSSHHHHHH SSGLVPRGSH MGSMAMACRQ EPQPQGPPPA AGAVASYDYL VIGGGSGGLA SARRAAELGA RAAVVESHKL GGTCVNVGCV PKKVMWNTAV HSEFMHDHAD YGFPSCEGKF NWRVIKEKRD AYVSRLNAIY QNNLTKSHIE IIRGHAAFTS DPKPTIEVSG KKYTAPHILI ATGGMPSTPH ESQIPGASLG ITSDGFFQLE ELPGRSVIVG AGYIAVEMAG ILSALGSKTS LMIRHDKVLR SFDSMISTNC TEELENAGVE VLKFSQVKEV KKTLSGLEVS MVTAVPGRLP VMTMIPDVDC LLWAIGRVPN TKDLSLNKLG IQTDDKGHII VDEFQNTNVK GIYAVGDVCG KALLTPVAIA AGRKLAHRLF EYKEDSKLDY NNIPTVVFSH PPIGTVGLTE DEAIHKYGIE NVKTYSTSFT PMYHAVTKRK TKCVMKMVCA NKEEKVVGIH MQGLGCDEML QGFAVAVKMG ATKADFDNTV AIHPTSSEEL VTLR