Recombinant humaan Glutathion S-transferase alfa 1/GSTA1-eiwit

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Glutathione S transferase A1, Glutathione S-transferase alpha 1, GST epsilon, GST2, GSTA1, GSTA1-1, GTH1, HA subunit 1

E.coli

Human

Non-Tagged

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 35,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

25.6 kDa (222aa) confirmed by MALDI-TOF

Glutathione S-transferase a1, GSTA1, Glutathione S-transferase alpha 1, GST2, GSTA1-1, GTH1, Glutathione S-transferase alpha 1 Glutathione S transferase A1, GST epsilon, GSTA1, GSTA1 1, HA subunit 1., ATGP0443-10 µg, ATGP0443-20 µg, ATGP0443-50 µg, ATGP0443-100 µg, ATGP0443-250 µg, ATGP0443-500 µg, ATGP0443-1 mg, ATGP0443-10, ATGP0443-20, ATGP0443-50, ATGP0443-100, ATGP0443-250, ATGP0443-500, ATGP0443-1

Rossi D., et al. (2009) Leukemia. 23 (6) :1118-26. ; ; Hou L., et al. (2007) J Biol Chem. 282 (32) :23264-74.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Enzymes & Proteases

NP_665683

P08263

Human

MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF