Recombinant humaan Glutathion S-transferase Mu 5/GSTM5 eiwit

Glutathione S-transferase mu 5, also known as GSTM5, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. GSTM5 plays an important role in detoxification. Recombinant human GSTM5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 90,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Glutathione S-transferase Mu 5, GSTM5-5, GTM5

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 90,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

28.2 kDa (242aa) confirmed by MALDI-TOF

GSTM5, Glutathione S-transferase Mu 5, GSTM5-5, GTM5, ATGP3142-10 µg, ATGP3142-20 µg, ATGP3142-50 µg, ATGP3142-100 µg, ATGP3142-250 µg, ATGP3142-500 µg, ATGP3142-1 mg, ATGP3142-10, ATGP3142-20, ATGP3142-50, ATGP3142-100, ATGP3142-250, ATGP3142-500, ATGP3142-1

Wang Y., et al. (2009) J Hum Genet. 54 (5) :271-6.; ; Hayes JD., et al. (1995) Crit Rev Biochem Mol Biol. 30 (6) : 445-600.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Signal Transduction

NP_000842

P46439

Human

MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQW LNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCY DPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV DFLAYDVLDM KRIFEPKCLD AFLNLKDFIS RFEGLKKISA YMKSSQFLRG LLFGKSATWN SK