Recombinant humaan Glutathion S-Transferase pi 1/GSTP1-eiwit

GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur) . The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 80 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

SERPINF1, Serpin peptidase inhibitor clade F member 1, Proliferation inducing protein 35, Pigment epithelium-derived factor EPC 1, Pigment epithelium-derived factor, PIG35, PI, GSTP1, GST3, Glutathione S-Transferase Pi 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S-transferase P, Glutathione S Transferase Pi, FAEES3, EPC1, DFN7, APF0619

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 80unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

27.4 kDa (246aa) confirmed by MALDI-TOF

EPC1, EPC-1, Glutathione S-Transferase pi1, GSTP1, IL 1 A, IL 1 alpha, IL 1A, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1alpha, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, PEDF, PIG 35, PIG35, Pigment epithelium derived factor, Pigment epithelium derived factor EPC 1, Pigment epithelium-derived factor, Pigment epithelium-derived factor EPC 1, Proliferation inducing protein 35, Serpin F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, SERPINF1, SERPIN-F1, ATGP0341-10 µg, ATGP0341-20 µg, ATGP0341-50 µg, ATGP0341-100 µg, ATGP0341-250 µg, ATGP0341-500 µg, ATGP0341-1 mg, ATGP0341-010, ATGP0341-020, ATGP0341-050, ATGP0341-100, ATGP0341-250, ATGP0341-500, ATGP0341-01M

Lee KA, et al. (2001) Blood. 98 (12) :3483-5.; ; Hayes JD, et al. (1995) Crit Rev Biochem Mol Biol. 30 (6) :445-600.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.0) containing 30% glycerol, 1mM EDTA, 0.1mM PMSF.

Enzymes & Proteases

NP_000843

P09211

Human

MPPY TVVYFPVRGR CAALRMLLAD QGQSWKEEVV TVETWQEGSL KASCLYGQLP KFQDGDLTLY QSNTILRHLG RTLGLYGKDQ QEAALVDMVN DGVEDLRCKY ISLIYTNYEA GKDDYVKALP GQLKPFETLL SQNQGGKTFI VGDQISFADY NLLDLLLIHE VLAPGCLDAF PLLSAYVGRL SARPKLKAFL ASPEYVNLPI NGNGKQ