Recombinant humaan Glutathion S-transferase thèta 1/GSTT1-eiwit

Glutathione S-transferase theta 1 (GSTT1) is one of the GSTs' four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e. g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Glutathione S-transferase theta 1, GSTT1, GST class-theta-1, Glutathione transferase T1-1, Glutathione S-transferase theta 1 EC 2.5.1.18, Glutathione S transferase 5, Glutathione S transferase theta 1, GST class theta 1, Glutathione transferase T1 1, GST 5 5, GST CL1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.5 kDa (277aa) confirmed by MALDI-TOF

Glutathione S-transferase theta1, GSTT1, Glutathione S-transferase theta 1, Glutathione S-transferase theta 1, GSTT1, GST class-theta-1, Glutathione transferase T1-1, Glutathione S-transferase theta 1 EC 2.5.1.18, Glutathione S transferase 5, Glutathione S transferase theta 1, GST class theta 1, Glutathione transferase T1 1, GST 5 5, GST CL1, ATGP0346-10 µg, ATGP0346-20 µg, ATGP0346-50 µg, ATGP0346-100 µg, ATGP0346-250 µg, ATGP0346-500 µg, ATGP0346-1 mg, ATGP0346-10, ATGP0346-20, ATGP0346-50, ATGP0346-100, ATGP0346-250, ATGP0346-500, ATGP0346-1

Faramawy MM., et al. (2009) . J Crit Care. 24 (3) :e7-10.; ; Delpisheh A., et al. (2009) . Eur J Obstet Gynecol Reprod Biol. 143 (1) :38-42.;

Toyoda Y, et al. Spontaneous Production of Glutathione-Conjugated Forms of 1,2-Dichloropropane: Comparative Study on Metabolic Activation Processes of Dihaloalkanes Associated with Occupational Cholangiocarcinoma. (Oxid Med Cell Longev. 2017) {https://pubmed.ncbi.nlm.nih.gov/28555163/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

AAH07065.1

P30711

Human

MGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR