Recombinant humaan GLYAT-eiwit

GLYAT, also known as glycine-N-acyltransferase, is a mitochondrial acyltransferase which conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Recombinant human GLYAT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Glycine-N-acyltransferase, ACGNAT, CAT, GAT

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.0 kDa (316aa) confirmed by MALDI-TOF

GLYAT, Glycine-N-acyltransferase, ACGNAT, CAT, GAT, ATGP1119-10 µg, ATGP1119-20 µg, ATGP1119-50 µg, ATGP1119-100 µg, ATGP1119-250 µg, ATGP1119-500 µg, ATGP1119-1 mg, ATGP1119-10, ATGP1119-20, ATGP1119-50, ATGP1119-100, ATGP1119-250, ATGP1119-500, ATGP1119-1

Mawal Y.R., et al. (1994) Biochem. Biophys. Res. Commun. 205:1373-1379; ; Westhuizen F.H., et al. (2000) J. Biochem. Mol. Toxicol. 14:102-109

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Metabolism

NP_964011

Q6IB77

Human

MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPSGGKP KAINQEMFKL SSMDVTHAHL VNKFWHFGGN ERSQRFIERC IQTFPTCCLL GPEGTPVCWD LMDQTGEMRM AGTLPEYRLH GLVTYVIYSH AQKLGKLGFP VYSHVDYSNE AMQKMSYTLQ HVPIPRSWNQ WNCVPL