Recombinant humaan glyatl2-eiwit

GLYATL2 belongs to the glycine N-acyltransferase family. This protein is expressed at highest levels in salivary gland and trachea. It is a mitochondrial acyltransferase which transfers the acyl group to the N-terminus of glycine. GLYATL2 conjugates numerous substrates, such as arachidonoyl-CoA and saturated medium and long-chain acyl-CoAs ranging from chain-length C8:0-CoA to C18:0-CoA, to form a variety of N-acylglycines. Recombinant human GLYATL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycine N-acyltransferase-like protein 2, glycine N-acyltransferase-like protein 2, BXMAS2-10, GATF-B

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.7 kDa (317aa) confirmed by MALDI-TOF

GLYATL2, glycine N-acyltransferase-like protein 2, glycine N-acyltransferase-like protein 2, BXMAS2-10, GATF-B, ATGP2551-10 µg, ATGP2551-20 µg, ATGP2551-50 µg, ATGP2551-100 µg, ATGP2551-250 µg, ATGP2551-500 µg, ATGP2551-1 mg, ATGP2551-10, ATGP2551-20, ATGP2551-50, ATGP2551-100, ATGP2551-250, ATGP2551-500, ATGP2551-1

Waluk, D.P., et al. (2012) J. Biol. Chem. 287 (20), 16158-16167; ; Matsuo, M., et al. (2012) Biochem. Biophys. Res. Commun. 420 (4), 901-906 ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 50% glycerol, 2mM DTT

Metabolism

NP_659453

Q8WU03

Human

MGSSHHHHHH SSGLVPRGSH MGSMLVLHNS QKLQILYKSL EKSIPESIKV YGAIFNIKDK NPFNMEVLVD AWPDYQIVIT RPQKQEMKDD QDHYTNTYHI FTKAPDKLEE VLSYSNVISW EQTLQIQGCQ EGLDEAIRKV ATSKSVQVDY MKTILFIPEL PKKHKTSSND KMELFEVDDD NKEGNFSNMF LDASHAGLVN EHWAFGKNER SLKYIERCLQ DFLGFGVLGP EGQLVSWIVM EQSCELRMGY TVPKYRHQGN MLQIGYHLEK YLSQKEIPFY FHVADNNEKS LQALNNLGFK ICPCGWHQWK CTPKKYC