Recombinant humaan Glycine N-methyltransferase/GNMT-eiwit

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC) . Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

Glycine N-methyltransferase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.

34.9 kDa (315aa) confirmed by MALDI-TOF

Glycine N-methyltransferase, GNMT, Glycine N-methyltransferase, ATGP3679-10 µg, ATGP3679-20 µg, ATGP3679-50 µg, ATGP3679-100 µg, ATGP3679-250 µg, ATGP3679-500 µg, ATGP3679-1 mg, ATGP3679-10, ATGP3679-20, ATGP3679-50, ATGP3679-100, ATGP3679-250, ATGP3679-500, ATGP3679-1

Liao YJ., et al. (2009) Int J Cancer.124 (4) :816-26.; ; Huang YC., et al. (2008) J Gastroenterol Hepatol. 23 (9) :1384-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Enzymes & Proteases

NP_061833

Q14749

Human

MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD