Recombinant humaan glycogenine-1-eiwit

Glycogenin 1 is an enzyme involved in glycogen biosynthesis. Glycogenin 1 is important for the function of self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. Also, it plays a role in regulating glycogen metabolism and the attainment of maximal glycogen levels in skeletal muscle. Recombinant human Glycogenin 1, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glycogenin, GYG1, GYG, Glycogenin1, GYG 1.

E.coli

Human

His-T7-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

41.2 kDa (365aa) confirmed by MALDI-TOF

Glycogenin1, Glycogenin, GYG1, GYG, Glycogenin1, GYG 1., ATGP0349-10 µg, ATGP0349-20 µg, ATGP0349-50 µg, ATGP0349-100 µg, ATGP0349-250 µg, ATGP0349-500 µg, ATGP0349-1 mg, ATGP0349-10, ATGP0349-20, ATGP0349-50, ATGP0349-100, ATGP0349-250, ATGP0349-500, ATGP0349-1

Yamashita H., et al. (1999) J Biol Chem. 274 (42) :29897-904. ; ; Declercg JP., et al. (2001) J Mol Biol. 311 (4) :751-9.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Metabolism

AAH00033

P46976

Human

MHHHHHHMAS MTGGQQMGRD LYDDDDKDRW GSMTDQAFVT LTTNDAYAKG ALVLGSSLKQ HRTTRRLVVL ATPQVSDSMR KVLETVFDEV IMVDVLDSGD SAHLTLMKRP ELGVTLTKLH CWSLTQYSKC VFMDADTLVL ANIDDLFDRE ELSAAPDPGW PDCFNSGVFV YQPSVETYNQ LLHLASEQGS FDGGDQGILN TFFSSWATTD IRKHLPFIYN LSSISIYSYL PAFKVFGASA KVVHFLGRVK PWNYTYDPKT KSVKSEAHDP NMTHPEFLIL WWNIFTTNVL PLLQQFGLVK DTCSYVNVED VSGAISHLSL GEIPAMAQPF VSSEERKERW EQGQADYMGA DSFDNIKRKL DTYLQ