Recombinant humaan glycophorine A/GYPA-eiwit

GYPA, also known as glycophorin-A isoform 1, is the major transmembrane sialoglycoprotein of red blood cells. It has been shown to contribute to the expression of the MN and Wright blood group antigens, to act as a receptor for the malaria parasite Plasmodium falciparum and Sendai virus, and along with the anion transporter, band 3, may contribute to the mechanical properties of the red blood cell membrane. It expression is locally increased in carotid atherosclerotic lesions of symptomatic compared to asymptomatic patients. Recombinant human GYPA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Glycophorin-A isoform 1, GYPA, CD235a, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta (C), MN, MNS, PAS-2, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

9.1 kDa (81aa)

Glycophorin A, Glycophorin-A isoform 1, GYPA, CD235a, GPA, GPErik, GPSAT, HGpMiV, HGpMiXI, HGpSta (C), MN, MNS, PAS-2, ATGP3592-10 µg, ATGP3592-20 µg, ATGP3592-50 µg, ATGP3592-100 µg, ATGP3592-250 µg, ATGP3592-500 µg, ATGP3592-1 mg, ATGP3592-10, ATGP3592-20, ATGP3592-50, ATGP3592-100, ATGP3592-250, ATGP3592-500, ATGP3592-1

Hassoun H., et al. (1998) Blood. 91:2146-2151.; ; Hakimi M., et al. (2013) Int J Mol Med. 32:331-338.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_002090

P02724

Human

Ala

LSTTEVA MHTSTSSSVT KSYISSQTND THKRDTYAAT PRAHEVSEIS VRTVYPPEEE TGERVQLAHH FSEPE