Recombinant humaan glycophorine C/GYPC-eiwit

GYPC, also known as glycophorin-C isoform 1, is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. Recombinant human GYPC protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Glycophorin-C isoform 1, GYPC, CD236, CD236R, GE, GPC, GPD, GYPD, PAS-2, PAS-2, Glycoconnectin, Glycophorin-D, GPD, Glycoprotein beta, PAS-2', Sialoglycoprotein D, GLPC

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

7.2 kDa (66aa)

GYPC, Glycophorin-C isoform 1, GYPC, CD236, CD236R, GE, GPC, GPD, GYPD, PAS-2, PAS-2, ATGP3547-10 µg, ATGP3547-20 µg, ATGP3547-50 µg, ATGP3547-100 µg, ATGP3547-250 µg, ATGP3547-500 µg, ATGP3547-1 mg, ATGP3547-10, ATGP3547-20, ATGP3547-50, ATGP3547-100, ATGP3547-250, ATGP3547-500, ATGP3547-1

Daniels G., et al. (1993) Blood. 82:3198-3203.; ; Wilder JA., et al. (2009) Mol Biol Evol. 26:2679-2687.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_002092.1

P04921

Human

Ala

MWSTRSP NSTAWPLSLE PDPGMASAST TMHTTTIAEP DPGMSGWPDG RMETSTPTIM