Recombinant humaan Glyoxalase II-eiwit

HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 500 units/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.

Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1

E.coli

Human

His-Tag

Enzyme Activity, SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 500unit/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis (2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.

31.4 kDa (284aa) confirmed by MALDI-TOF

Glyoxalase II, Hydroxyacylglutathione hydrolase, GLO, GLX2, Glyoxalase II, HAGH1, ATGP0808-10 µg, ATGP0808-20 µg, ATGP0808-50 µg, ATGP0808-100 µg, ATGP0808-250 µg, ATGP0808-500 µg, ATGP0808-1 mg, ATGP0808-10, ATGP0808-20, ATGP0808-50, ATGP0808-100, ATGP0808-250, ATGP0808-500, ATGP0808-1

Vila AJ., et al. (2010) J Inorg Biochem. 104 (7) :726-31.; ; Crowder MW., et al (2009) Biochemistry. 48 (23) :5426-34.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol

Enzymes & Proteases

NP_001035517

Q16775

Human

MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD