Recombinant humaan GM-CSF R alfa-eiwit

CSF2RA, also known as granulocyte-macrophage colony-stimulating factor receptor subunit alpha isoform g, is a well-known hemopoietic growth factor that is used in the clinic to correct neutropaenia, usually as a result of chemotherapy. It is a key regulatory molecule causative of this aortopathy, and modulation of this cytokine might be an exploitable treatment strategy for the condition. It transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. Recombinant human CSF2RA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤10 µg/mL with Human GM-CSF.

Granulocyte-macrophage colony-stimulating factor receptor subunit alpha isoform g, CSF2RA, CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMR, SMDP4, alphaGMR

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤10 µg/mL with Human GM-CSF.

35.9 kDa (310aa)

GM-CSF R a, ATGP3697-10 µg, ATGP3697-20 µg, ATGP3697-50 µg, ATGP3697-100 µg, ATGP3697-250 µg, ATGP3697-500 µg, ATGP3697-1 mg, ATGP3697-10, ATGP3697-20, ATGP3697-50, ATGP3697-100, ATGP3697-250, ATGP3697-500, ATGP3697-1

Van Nieuwenhuijze A., et al. (2013) Mol Immunol. 56:675-682.; ; Raines MA., et al. (1991) Proc Natl Acad Sci U S A. 88:8203-8207.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_001155003

P15509

Human

Ala

LIPEKSD LRTVAPASSL NVRFDSRTMN LSWDCQENTT FSKCFLTDKK NRVVEPRLSN NECSCTFREI CLHEGVTFEV HVNTSQRGFQ QKLLYPNSGR EGTAAQNFSC FIYNADLMNC TWARGPTAPR DVQYFLYIRN SKRRREIRCP YYIQDSGTHV GCHLDNLSGL TSRNYFLVNG TSREIGIQFF DSLLDTKKIE RFNPPSNVTV RCNTTHCLVR WKQPRTYQKL SYLDFQYQLD VHRKNTQPGT ENLLINVSGD LENRYNFPSS EPRAKHSVKI RAADVRILNW SSWSEAIEFG SDDG