Recombinant humaan GM3 Synthase/ST3GAL5 eiwit

Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. Recombinant human ST3GAL5 protein, fused to His-tag at N-terminus, was expressed in E. coli.

"ST3 beta-galactoside alpha-2,3-sialyltransferase 5", "Lactosylceramide alpha-2,3-sialyltransferase", "CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase", GM3 synthase, Ganglioside GM3 synthase, ST3Gal V, Sialyltransferase 9, SIAT9

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

41.0 kDa (359aa)

"CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase", "Lactosylceramide alpha-2,3-sialyltransferase", "ST3 beta-galactoside alpha-2,3-sialyltransferase 5", 3-sialyltransferase 5, 3-sialyltransferase isoform 1, Ganglioside GM3 synthase, GM3 synthase, Lactosylceramide alpha-2, Lactosylceramide alpha-23-sialyltransferase isoform 1, SATI, Sialyltransferase 9, SIAT9, SIATGM3S, ST3 beta-galactoside alpha-2, ST3Gal V, ST3GAL5, ST3GalV, ATGP2418-10 µg, ATGP2418-20 µg, ATGP2418-50 µg, ATGP2418-100 µg, ATGP2418-250 µg, ATGP2418-500 µg, ATGP2418-1 mg, ATGP2418-010, ATGP2418-020, ATGP2418-050, ATGP2418-100, ATGP2418-250, ATGP2418-500, ATGP2418-01M

Berselli P., Zava S.. et al. (2006) . Biochim. Biophys. Acta 1759:348-358

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Signal Transduction

NP_003887

Q9UNP4

Human

LKLNYTT EECDMKKMHY VDPDHVKRAQ KYAQQVLQKE CRPKFAKTSM ALLFEHRYSV DLLPFVQKAP KDSEAESKYD PPFGFRKFSS KVQTLLELLP EHDLPEHLKA KTCRRCVVIG SGGILHGLEL GHTLNQFDVV IRLNSAPVEG YSEHVGNKTT IRMTYPEGAP LSDLEYYSND LFVAVLFKSV DFNWLQAMVK KETLPFWVRL FFWKQVAEKI PLQPKHFRIL NPVIIKETAF DILQYSEPQS RFWGRDKNVP TIGVIAVVLA THLCDEVSLA GFGYDLNQPR TPLHYFDSQC MAAMNFQTMH NVTTETKFLL KLVKEGVVKD LSGGIDREF