Recombinant humaan GMDS-eiwit

GMDS, also known as SDR3E1, belongs to the GDP-mannose 4, 6-dehydratase family. This protein utilizes NADP as a cofactor to catalyze the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose. Mutations in the gene encoding GMDS are involved in resistance to TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) -induced apoptosis. Recombinant human GMDS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

GDP-mannose 46 dehydratase, GDP-mannose 4,6 dehydratase, GMD, SDR3E1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

44.1 kDa (392aa) confirmed by MALDI-TOF

GMDS, ATGP1282-10 µg, ATGP1282-20 µg, ATGP1282-50 µg, ATGP1282-100 µg, ATGP1282-250 µg, ATGP1282-500 µg, ATGP1282-1 mg, ATGP1282-10, ATGP1282-20, ATGP1282-50, ATGP1282-100, ATGP1282-250, ATGP1282-500, ATGP1282-1

Ohyama C. et al. (1998) J Biol Chem. 273: 14582-14587.; ; Moriwaki K. et al. (2009) Gastroenterology 137: 188-198.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF

Metabolism

NP_001491

O60547

Human

MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH ADVELMRTNP NA