Recombinant humaan GMP-synthase/GMPS-eiwit

GMP synthase, also known as GMPS, is involved in purine biosynthesis. Existing as a homodimer, GMPS catalyzes the last step in the GMP synthesis pathway, namely the ATP-dependent amination of XMP to GMP. GMPS contains one GMP-binding domain and one glutamine amidotransferase type-1 domain through which it conveys its catalytic activity. Chromosomal translocations involving the gene encoding GMPS are associated with acute myeloid leukemias, suggesting a possible role for GMPS in carcinogenesis. Recombinant human GMPS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GMP synthase [glutamine-hydrolyzing], Glutamine amidotransferase, guanine monophosphate synthetase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

79.2 kDa (717aa)

GMPS, GMP synthase [glutamine-hydrolyzing], Glutamine amidotransferase, guanine monophosphate synthetase, ATGP1403-10 µg, ATGP1403-20 µg, ATGP1403-50 µg, ATGP1403-100 µg, ATGP1403-250 µg, ATGP1403-500 µg, ATGP1403-1 mg, ATGP1403-10, ATGP1403-20, ATGP1403-50, ATGP1403-100, ATGP1403-250, ATGP1403-500, ATGP1403-1

Hirst M., et al. (1994) J Biol Chem. 269:23830-23837.; ; Nakamura J., et al. (1995) J Biol Chem. 270:23450-23455.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Metabolism

NP_003866

P49915

Human

MALCNG DSKLENAGGD LKDGHHHYEG AVVILDAGAQ YGKVIDRRVR ELFVQSEIFP LETPAFAIKE QGFRAIIISG GPNSVYAEDA PWFDPAIFTI GKPVLGICYG MQMMNKVFGG TVHKKSVRED GVFNISVDNT CSLFRGLQKE EVVLLTHGDS VDKVADGFKV VARSGNIVAG IANESKKLYG AQFHPEVGLT ENGKVILKNF LYDIAGCSGT FTVQNRELEC IREIKERVGT SKVLVLLSGG VDSTVCTALL NRALNQEQVI AVHIDNGFMR KRESQSVEEA LKKLGIQVKV INAAHSFYNG TTTLPISDED RTPRKRISKT LNMTTSPEEK RKIIGDTFVK IANEVIGEMN LKPEEVFLAQ GTLRPDLIES ASLVASGKAE LIKTHHNDTE LIRKLREEGK VIEPLKDFHK DEVRILGREL GLPEELVSRH PFPGPGLAIR VICAEEPYIC KDFPETNNIL KIVADFSASV KKPHTLLQRV KACTTEEDQE KLMQITSLHS LNAFLLPIKT VGVQGDCRSY SYVCGISSKD EPDWESLIFL ARLIPRMCHN VNRVVYIFGP PVKEPPTDVT PTFLTTGVLS TLRQADFEAH NILRESGYAG KISQMPVILT PLHFDRDPLQ KQPSCQRSVV IRTFITSDFM TGIPATPGNE IPVEVVLKMV TEIKKIPGIS RIMYDLTSKP PGTTEWE