Recombinant humaan GNAI1-eiwit

GNAI1, also known as guanine nucleotide-binding protein G (i) subunit alpha, functions to relay information from cell surface receptors to intracellular effectors. Also this protein functions to open atrial potassium channels. Recombinant human GNAI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Guanine nucleotide-binding protein G (i) subunit alpha, Gi

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

42.7 kDa (377aa) confirmed by MALDI-TOF

GNAI1, Guanine nucleotide-binding protein G (i) subunit alpha, Gi, ATGP1224-10 µg, ATGP1224-20 µg, ATGP1224-50 µg, ATGP1224-100 µg, ATGP1224-250 µg, ATGP1224-500 µg, ATGP1224-1 mg, ATGP1224-10, ATGP1224-20, ATGP1224-50, ATGP1224-100, ATGP1224-250, ATGP1224-500, ATGP1224-1

Jones D.T. et al. (1990) J Biol Chem. 265: 2671-2676.; ; Simon M.I. et al. (1991) Science 252: 802-808.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Signal Transduction

NP_002060.4

P63096

Human

MGCTLSA EDKAAVERSK MIDRNLREDG EKAAREVKLL LLGAGESGKS TIVKQMKIIH EAGYSEEECK QYKAVVYSNT IQSIIAIIRA MGRLKIDFGD SARADDARQL FVLAGAAEEG FMTAELAGVI KRLWKDSGVQ ACFNRSREYQ LNDSAAYYLN DLDRIAQPNY IPTQQDVLRT RVKTTGIVET HFTFKDLHFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTDTSIILF LNKKDLFEEK IKKSPLTICY PEYAGSNTYE EAAAYIQCQF EDLNKRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKDCGLF