Recombinant humaan GNAI2-eiwit

GNAI2 is an alpha subunit of guanine nucleotide binding proteins (G proteins) . The protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only two are known so far. Recombinant human GNAI2 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Guanine nucleotide-binding protein G (i) subunit alpha-2, GIP, GNAI2B, H_LuCA15.1, H_LuCA16.1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42 kDa (375aa) confirmed by MALDI-TOF

GNAI2, Guanine nucleotide-binding protein G (i) subunit alpha-2, GIP, GNAI2B, H_LuCA15.1, H_LuCA16.1, ATGP1720-10 µg, ATGP1720-20 µg, ATGP1720-50 µg, ATGP1720-100 µg, ATGP1720-250 µg, ATGP1720-500 µg, ATGP1720-1 mg, ATGP1720-10, ATGP1720-20, ATGP1720-50, ATGP1720-100, ATGP1720-250, ATGP1720-500, ATGP1720-1

Magovcevic I, Ang SL, et al. (1992) . Genomics. 12 (1) :125-9.; ; Damaj BB, McColl SR, et al. (1996) . FASEB J. Oct; ;10 (12) :1426-34.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_002061

P04899

Human

MGCTVSAEDK AAAERSKMID KNLREDGEKA AREVKLLLLG AGESGKSTIV KQMKIIHEDG YSEEECRQYR AVVYSNTIQS IMAIVKAMGN LQIDFADPSR ADDARQLFAL SCTAEEQGVL PDDLSGVIRR LWADHGVQAC FGRSREYQLN DSAAYYLNDL ERIAQSDYIP TQQDVLRTRV KTTGIVETHF TFKDLHFKMF DVGGQRSERK KWIHCFEGVT AIIFCVALSA YDLVLAEDEE MNRMHESMKL FDSICNNKWF TDTSIILFLN KKDLFEEKIT HSPLTICFPE YTGANKYDEA ASYIQSKFED LNKRKDTKEI YTHFTCATDT KNVQFVFDAV TDVIIKNNLK DCGLF