Recombinant humaan GNAI3-eiwit

GNAI3 belongs to the G-alpha family and G (i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G (k) is the stimulatory G protein of receptor-regulated K+ channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. This protein plays a role in cell division. GNAI3 has been shown to interact with RGS14, RIC8A, RGS18, S1PR1, RGS12, RGS16, RGS19, RGS10 and RGS5. Recombinant human GNAI3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Guanine nucleotide-binding protein G (k) subunit alpha, 87u6

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

43 kDa (377aa) confirmed by MALDI-TOF

GNAI3, Guanine nucleotide-binding protein G (k) subunit alpha, 87u6, ATGP1486-10 µg, ATGP1486-20 µg, ATGP1486-50 µg, ATGP1486-100 µg, ATGP1486-250 µg, ATGP1486-500 µg, ATGP1486-1 mg, ATGP1486-10, ATGP1486-20, ATGP1486-50, ATGP1486-100, ATGP1486-250, ATGP1486-500, ATGP1486-1

Cho H., et al. (2007) J. Cell Biol. 178:245-255; ; Jones, D.T., et al. (1990) J. Biol. Chem. 265: 2671-2676.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl

Signal Transduction

NP_006487

P08754

Human

MGSSHHHHHH SSGLVPRGSH MGSMGCTLSA EDKAAVERSK MIDRNLREDG EKAAKEVKLL LLGAGESGKS TIVKQMKIIH EDGYSEDECK QYKVVVYSNT IQSIIAIIRA MGRLKIDFGE AARADDARQL FVLAGSAEEG VMTPELAGVI KRLWRDGGVQ ACFSRSREYQ LNDSASYYLN DLDRISQSNY IPTQQDVLRT RVKTTGIVET HFTFKDLYFK MFDVGGQRSE RKKWIHCFEG VTAIIFCVAL SDYDLVLAED EEMNRMHESM KLFDSICNNK WFTETSIILF LNKKDLFEEK IKRSPLTICY PEYTGSNTYE EAAAYIQCQF EDLNRRKDTK EIYTHFTCAT DTKNVQFVFD AVTDVIIKNN LKECGLY