Recombinant humaan GNB1-eiwit

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. GNB1 encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. Recombinant human GNB1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1, guanine nucleotide binding protein (G protein), beta polypeptide 1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

39.8 kDa (363aa)

GNB1, guanine nucleotide-binding protein G (I) /G (S) /G (T) subunit beta-1, guanine nucleotide binding protein (G protein), beta polypeptide 1, ATGP2665-10 µg, ATGP2665-20 µg, ATGP2665-50 µg, ATGP2665-100 µg, ATGP2665-250 µg, ATGP2665-500 µg, ATGP2665-1 mg, ATGP2665-10, ATGP2665-20, ATGP2665-50, ATGP2665-100, ATGP2665-250, ATGP2665-500, ATGP2665-1

Bhatnagar, A, et al. (2013) PLoS ONE 8 (1), E52689 ; ; Lim, Y.P., et al. (2012) BMC Gastroenterol 12, 167;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M UREA, 10% glycerol

Signal Transduction

NP_002065

P62873

Human

MSELDQL RQEAEQLKNQ IRDARKACAD ATLSQITNNI DPVGRIQMRT RRTLRGHLAK IYAMHWGTDS RLLVSASQDG KLIIWDSYTT NKVHAIPLRS SWVMTCAYAP SGNYVACGGL DNICSIYNLK TREGNVRVSR ELAGHTGYLS CCRFLDDNQI VTSSGDTTCA LWDIETGQQT TTFTGHTGDV MSLSLAPDTR LFVSGACDAS AKLWDVREGM CRQTFTGHES DINAICFFPN GNAFATGSDD ATCRLFDLRA DQELMTYSHD NIICGITSVS FSKSGRLLLA GYDDFNCNVW DALKADRAGV LAGHDNRVSC LGVTDDGMAV ATGSWDSFLK IWN