Recombinant humaan GNG13-eiwit

Heterotrimeric G proteins, which consist of alpha, beta, and gamma subunits, function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. GNG13 is a gamma subunit that is expressed in taste, retinal, and neuronal tissues and plays a key role in taste transduction Recombinant human GNG13 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Guanine nucleotide binding protein (G protein) gamma 13, Guanine nucleotide binding protein (G protein), gamma 13, G (gamma) 13, h2-35

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

10 kDa (87aa) confirmed by MALDI-TOF

GNG13, Guanine nucleotide binding protein (G protein) gamma 13, Guanine nucleotide binding protein (G protein), gamma 13, G (gamma) 13, h2-35, ATGP2788-10 µg, ATGP2788-20 µg, ATGP2788-50 µg, ATGP2788-100 µg, ATGP2788-250 µg, ATGP2788-500 µg, ATGP2788-1 mg, ATGP2788-10, ATGP2788-20, ATGP2788-50, ATGP2788-100, ATGP2788-250, ATGP2788-500, ATGP2788-1

Huang L., et al. (1999) Nat. Neurosci. 2:1055-1062; ; Martin J., et al. (2004) Nature. 432:988-994

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT, 0.1mM PMSF

Signal Transduction

NP_057625

Q9P2W3

Human

MGSSHHHHHH SSGLVPRGSH MGSMEEWDVP QMKKEVESLK YQLAFQREMA SKTIPELLKW IEDGIPKDPF LNPDLMKNNP WVEKGKC