Recombinant humaan GNNF-eiwit

GDNF, also known as Glial cell-derived neurotrophic factor, is a member of the GDNF family of ligands (GFL) . This Protein has been found that GFLs play a role in a number of biological processes including cell survival, neurite outgrowth, cell differentiation and cell migration. It promotes the survival of many types of neurons and signals through GFRa receptors, particularly GFRa1. These neuronal populations die in the course of Parkinson's disease and amyotrophic lateral sclerosis (ALS) . GDNF also regulates kidney development and spermatogenesis, and has a powerful and rapid negative (ameliorating) effect on alcohol consumption. Recombinant human GDNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human GDNF is coated at 0.5 µg/mL (100 ul/well) can bind Human GFR alpha-1 (CAT# ATGP4154) in a Functional ELISA assay.

Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, ATF1, ATF2, HFB1-GDNF, HSCR3

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human GFR alpha-1 (CAT# ATGP4154) .

12.8kDa (113aa)

, ATGP3942-10 µg, ATGP3942-20 µg, ATGP3942-50 µg, ATGP3942-100 µg, ATGP3942-250 µg, ATGP3942-500 µg, ATGP3942-1 mg, ATGP3942-10, ATGP3942-20, ATGP3942-50, ATGP3942-100, ATGP3942-250, ATGP3942-500, ATGP3942-1

Lin LF., et al, (1993) Science. 260:1130–1132.; ; Carnicella S., et al, (2008) United States of America. 105: 8114–8119.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Immunology

NP_000505.1

P39905

Human

Ala

RGQRGK NRGCVLTAIH LNVTDLGLGY ETKEELIFRY CSGSCDAAET TYDKILKNLS RNRRLVSDKV GQACCRPIAF DDDLSFLDDN LVYHILRKHS AKRCGCI