Recombinant humaan GOLM1-eiwit

Golgi membrane protein 1, also known as GOLM1, is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Furthermore, because GOLM1 is so uniquely regulated in cells, it can be successfully used as a clinically relevant molecular biomarker for cancer. Recombinant human GOLM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Golgi membrane protein 1, bA379P1.3, C9orf155, GOLPH2, GP73, PSEC0257

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

44 kDa (389aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

GOLM1, Golgi membrane protein 1, bA379P1.3, C9orf155, GOLPH2, GP73, PSEC0257, ATGP2430-10 µg, ATGP2430-20 µg, ATGP2430-50 µg, ATGP2430-100 µg, ATGP2430-250 µg, ATGP2430-500 µg, ATGP2430-1 mg, ATGP2430-10, ATGP2430-20, ATGP2430-50, ATGP2430-100, ATGP2430-250, ATGP2430-500, ATGP2430-1

Kim HJ., et al. (2012) Cell Biosci. 2 (1) : 31.; ; Li L., et al. (2012) PLoS One. 7 (6) : 38939

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_808800

Q8NBJ4

Human

MGSSHHHHHH SSGLVPRGSH MGSSSRSVDL QTRIMELEGR VRRAAAERGA VELKKNEFQG ELEKQREQLD KIQSSHNFQL ESVNKLYQDE KAVLVNNITT GERLIRVLQD QLKTLQRNYG RLQQDVLQFQ KNQTNLERKF SYDLSQCINQ MKEVKEQCEE RIEEVTKKGN EAVASRDLSE NNDQRQQLQA LSEPQPRLQA AGLPHTEVPQ GKGNVLGNSK SQTPAPSSEV VLDSKRQVEK EETNEIQVVN EEPQRDRLPQ EPGREQVVED RPVGGRGFGG AGELGQTPQV QAALSVSQEN PEMEGPERDQ LVIPDGQEEE QEAAGEGRNQ QKLRGEDDYN MDENEAESET DKQAALAGND RNIDVFNVED QKRDTINLLD QREKRNHTL