Recombinant humaan GPD1-eiwit

GPD1, also known as glycerol-3-phosphate dehydrogenase, is an enzyme that catalyzes the reduction of dihydroxyacetone phosphate to sn-glycerol 3-phosphate. (sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH) Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase and glycerolphosphate dehydrogenase. However, GPD1 is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Recombinant GPD1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Glycerol-3-phosphate dehydrogenase [NAD+], Glycerol-3-phosphate dehydrogenase [NAD+] AI747587, EC 1.1.1.8, FLJ26652, G3PD, Gdc-1, Gdc1, Gdp1, Glycerol 3 phosphate dehydrogenase 1, Glycerol 3 phosphate dehydrogenase cytosolic, Glycerol 3 phosphate dehydrogenase soluble, Glycerol-3-phosphate dehydrogenase, Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic, Glycerol-3-phosphate dehydrogenase 1 (soluble), Glycerol-3-phosphate dehydrogenase, soluble, Glycerphosphate dehydrogenase, GPD-C, Gpd1 protein, GPD2, Gpd3, Gpdc, GPDH, GPDH-C, Gpdhc, KIAA4010, MGC93453, MGPD, mKIAA4010.

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.5 kDa (349aa) confirmed by MALDI-TOF

GPD1, Glycerol-3-phosphate dehydrogenase [NAD+], Glycerol-3-phosphate dehydrogenase [NAD+] AI747587, EC 1.1.1.8, FLJ26652, G3PD, Gdc-1, Gdc1, Gdp1, Glycerol 3 phosphate dehydrogenase 1, Glycerol 3 phosphate dehydrogenase cytosolic, Glycerol 3 phosphate dehydrogenase soluble, Glycerol-3-phosphate dehydrogenase, Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic, Glycerol-3-phosphate dehydrogenase 1 (soluble), Glycerol-3-phosphate dehydrogenase, soluble, Glycerphosphate dehydrogenase, GPD-C, Gpd1 protein, GPD2, Gpd3, Gpdc, GPDH, GPDH-C, Gpdhc, KIAA4010, MGC93453, MGPD, mKIAA4010., ATGP0486-10 µg, ATGP0486-20 µg, ATGP0486-50 µg, ATGP0486-100 µg, ATGP0486-250 µg, ATGP0486-500 µg, ATGP0486-1 mg, ATGP0486-10, ATGP0486-20, ATGP0486-50, ATGP0486-100, ATGP0486-250, ATGP0486-500, ATGP0486-1

Albertyn J., et al. (1992) FEBS Lett. 308 (2) : 130-2.; ; Ou X., et al. (2006) J. Mol. Biol. 357 (3) :858-69 ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Redox Proteins

NP_005267

P21695

Human

MASKKVCIVG SGNWGSAIAK IVGGNAAQLA QFDPRVTMWV FEEDIGGKKL TEIINTQHEN VKYLPGHKLP PNVVAVPDVV QAAEDADILI FVVPHQFIGK ICDQLKGHLK ANATGISLIK GVDEGPNGLK LISEVIGERL GIPMSVLMGA NIASEVADEK FCETTIGCKD PAQGQLLKEL MQTPNFRITV VQEVDTVEIC GALKNVVAVG AGFCDGLGFG DNTKAAVIRL GLMEMIAFAK LFCSGPVSSA TFLESCGVAD LITTCYGGRN RKVAEAFART GKSIEQLEKE LLNGQKLQGP ETARELYSIL QHKGLVDKFP LFMAVYKVCY EGQPVGEFIH CLQNHPEHM