Recombinant humaan GPI-eiwit

Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 400 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C.

GPI, AMF, GNPI, NLK, PGI, PHI, SA36

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 400unit/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will convert 1.0 umole of D-Fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 37C.

65.3 kDa (578aa) confirmed by MALDI-TOF

GPI, GPI, AMF, GNPI, NLK, PGI, PHI, SA36, ATGP3232-10 µg, ATGP3232-20 µg, ATGP3232-50 µg, ATGP3232-100 µg, ATGP3232-250 µg, ATGP3232-500 µg, ATGP3232-1 mg, ATGP3232-10, ATGP3232-20, ATGP3232-50, ATGP3232-100, ATGP3232-250, ATGP3232-500, ATGP3232-1

Lin HY., et al. (2009) Biochim Biophys Acta. 1794 (2) :315-23. ; ; Beutler E., et al. (1997) Blood cells Mol. 23:402-409

Tanie Y, et al. Extracellular Neuroleukin Enhances Neuroleukin Secretion From Astrocytes and Promotes Axonal Growth in vitro and in vivo. (Front Pharmacol. 2018) {https://pubmed.ncbi.nlm.nih.gov/30459611/}; ; Tanie Y, et al. GRP78-Mediated Signaling Contributes to Axonal Growth Resulting in Motor Function Recovery in Spinal Cord-Injured Mice. (Front Pharmacol. 2020) {https://pubmed.ncbi.nlm.nih.gov/32547396/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Enzymes & Proteases

NP_000166

P06744

Human

MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ