Recombinant humaan Granzyme B-eiwit

GZMB, also known as granzyme B isoform 1, is member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. Tis protein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. Also, it processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. Recombinant human GZMB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

Granzyme B isoform 1, GZMB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 2,500pmol/min/ug, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C.

26.5 kDa (235aa)

Granzyme B, Granzyme B isoform 1, GZMB, C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, ATGP3774-10 µg, ATGP3774-20 µg, ATGP3774-50 µg, ATGP3774-100 µg, ATGP3774-250 µg, ATGP3774-500 µg, ATGP3774-1 mg, ATGP3774-10, ATGP3774-20, ATGP3774-50, ATGP3774-100, ATGP3774-250, ATGP3774-500, ATGP3774-1

Poe M., et al, (1991) J. Biol. Chem. 266:98-103.; ; Xu W., et al, (2014) Eur. J. Immunol. 44 (1) 275-284.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Apoptosis

NP_004122.2

P10144

Human

Gly19