Recombinant humaan Granzyme K-eiwit

GZMK, as known as Granzyme K, is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. Recombinant human GZMK, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Granzyme K, GZMK, TRYP2, GZMK, PRSS, tryptase II, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

26.9 kDa (247aa)

GZMK, ATGP3539-10 µg, ATGP3539-20 µg, ATGP3539-50 µg, ATGP3539-100 µg, ATGP3539-250 µg, ATGP3539-500 µg, ATGP3539-1 mg, ATGP3539-10, ATGP3539-20, ATGP3539-50, ATGP3539-100, ATGP3539-250, ATGP3539-500, ATGP3539-1

Jiang W., et al, (2011) J. Immunol. 187:781-790.; ; Wensink AC., et al, (2014) Proc. Natl. Acad. Sci. U.S.A. 111:5974-5979.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Apoptosis

NP_002095.1

P49863

Human

Ala

IIGGKEV SPHSRPFMAS IQYGGHHVCG GVLIDPQWVL TAAHCQYRFT KGQSPTVVLG AHSLSKNEAS KQTLEIKKFI PFSRVTSDPQ SNDIMLVKLQ TAAKLNKHVK MLHIRSKTSL RSGTKCKVTG WGATDPDSLR PSDTLREVTV TVLSRKLCNS QSYYNGDPFI TKDMVCAGDA KGQKDSCKGD SGGPLICKGV FHAIVSGGHE CGVATKPGIY TLLTKKYQTW IKSNLVPPHT N