Recombinant humaan GRAP2-eiwit

GRAP2, also known as GADS, is a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Recombinant human GRAP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GRB2-related adapter protein 2, GADS, GRB2L, GRBLG, GrbX, Grf40, GRID, GRPL, Mona, P38

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

40 kDa (350aa) confirmed by MALDI-TOF

GRAP2, GRB2-related adapter protein 2, GADS, GRB2L, GRBLG, GrbX, Grf40, GRID, GRPL, Mona, P38, ATGP0888-10 µg, ATGP0888-20 µg, ATGP0888-50 µg, ATGP0888-100 µg, ATGP0888-250 µg, ATGP0888-500 µg, ATGP0888-1 mg, ATGP0888-10, ATGP0888-20, ATGP0888-50, ATGP0888-100, ATGP0888-250, ATGP0888-500, ATGP0888-1

Ludwig L., et al. (2009) Cancer Lett. 275 (2) :194-7.; ; Chang TW., et al. (2008) Oncogene. 27 (3) :332-8.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Cancer

NP_004801

O75791

Human

MGSSHHHHHH SSGLVPRGSH MEAVAKFDFT ASGEDELSFH TGDVLKILSN QEEWFKAELG SQEGYVPKNF IDIQFPKWFH EGLSRHQAEN LLMGKEVGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD NKGNYFLWTE KFPSLNKLVD YYRTNSISRQ KQIFLRDRTR EDQGHRGNSL DRRSQGGPHL SGAVGEEIRP SMNRKLSDHP PTLPLQQHQH QPQPPQYAPA PQQLQQPPQQ RYLQHHHFHQ ERRGGSLDIN DGHCGTGLGS EMNAALMHRR HTDPVQLQAA GRVRWARALY DFEALEDDEL GFHSGEVVEV LDSSNPSWWT GRLHNKLGLF PANYVAPMTR