Recombinant humaan Gremlin-eiwit

GREM1 is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this protein belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this protein may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Recombinant human GREM1 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Gremlin-1 isoform 1, CKTSF1B1, DAND2, DRM, GREMLIN, IHG-2, PIG2

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

20.7 kDa (183aa)

Gremlin, Gremlin-1 isoform 1, CKTSF1B1, DAND2, DRM, GREMLIN, IHG-2, PIG2, ATGP1961-10 µg, ATGP1961-20 µg, ATGP1961-50 µg, ATGP1961-100 µg, ATGP1961-250 µg, ATGP1961-500 µg, ATGP1961-1 mg, ATGP1961-10, ATGP1961-20, ATGP1961-50, ATGP1961-100, ATGP1961-250, ATGP1961-500, ATGP1961-1

Helena Stabile, Stefania Mitola, et al. (2007) . Blood 109:5 1834-1840.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Signal Transduction

NP_037504.1

O60565

Human

KKKGSQG AIPPPDKAQH NDSEQTQSPQ QPGSRNRGRG QGRGTAMPGE EVLESSQEAL HVTERKYLKR DWCKTQPLKQ TIHEEGCNSR TIINRFCYGQ CNSFYIPRHI RKEEGSFQSC SFCKPKKFTT MMVTLNCPEL QPPTKKKRVT RVKQCRCISI DLD