Recombinant humaan GRHPR-eiwit

GRHPR, also known as GLXR, is a member of the D-isomer specific 2-hydroxyacid dehydrogenase family of proteins. Localizing to the cytosol, GRHPR is ubiquitously expressed with highest expression levels found in liver. The protein has widespread tissue expression and has a role in metabolism. GRHPR is an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. Recombinant human GRHPR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Glyoxylate reductase/hydroxypyruvate reductase, GLXR, GLYD, PH2, Glyoxylate reductase/hydroxypyruvate reductase glycerate 2 dehydrogenase, Glyoxylate reductase/hydroxypyruvate reductase, OTTHuMP00000046131, PH 2, Primary hyperoxaluria type 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.8 kDa (348aa) confirmed by MALDI-TOF

GRHPR, Glyoxylate reductase/hydroxypyruvate reductase, GLXR, GLYD, PH2, Glyoxylate reductase/hydroxypyruvate reductase glycerate 2 dehydrogenase, Glyoxylate reductase/hydroxypyruvate reductase, OTTHuMP00000046131, PH 2, Primary hyperoxaluria type 2, ATGP0577-10 µg, ATGP0577-20 µg, ATGP0577-50 µg, ATGP0577-100 µg, ATGP0577-250 µg, ATGP0577-500 µg, ATGP0577-1 mg, ATGP0577-10, ATGP0577-20, ATGP0577-50, ATGP0577-100, ATGP0577-250, ATGP0577-500, ATGP0577-1

Booth MP., et al. (2006) J Mol Biol. 360 (1) :178-89. ; ; Genolet R., et al. (2005) J Biol Chem. 280 (25) :24143-52.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Metabolism

NP_036335

Q9UBQ7

Human

MRPVRLMKVF VTRRIPAEGR VALARAADCE VEQWDSDEPI PAKELERGVA GAHGLLCLLS DHVDKRILDA AGANLKVIST MSVGIDHLAL DEIKKRGIRV GYTPDVLTDT TAELAVSLLL TTCRRLPEAI EEVKNGGWTS WKPLWLCGYG LTQSTVGIIG LGRIGQAIAR RLKPFGVQRF LYTGRQPRPE EAAEFQAEFV STPELAAQSD FIVVACSLTP ATEGLCNKDF FQKMKETAVF INISRGDVVN QDDLYQALAS GKIAAAGLDV TSPEPLPTNH PLLTLKNCVI LPHIGSATHR TRNTMSLLAA NNLLAGLRGE PMPSELKL