Recombinant humaan groeihormoon R/GHR-eiwit

GHR, also known as growth hormone receptor, is a member of the cytokine receptor family. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, growth hormone acts as an antagonist because of a large difference in affinities at the respective binding sites. This antagonist action can be enhanced further by reducing binding in the low affinity binding site. Recombinant human GHR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human Growth Hormone R/GHR in inhibit the cell growth using Nb2-11 Rat lymphoma cells in the presence of 1.25 ng/mL of human growth hormone. The ED50 range ≤ 10 ng/mL.

GHR, GHBP, GHIP

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by ability to inhibit GH-induced proliferation assay using Nb2-11 Rat lymphoma cells in the presence of 1.25 ng/mL of human growth hormone. The ED50 range ≤ 10 ng/mL.

29.4 kDa (254aa)

Growth Hormone GHR, GHR, GHBP, GHIP, ATGP3207-10 µg, ATGP3207-20 µg, ATGP3207-50 µg, ATGP3207-100 µg, ATGP3207-250 µg, ATGP3207-500 µg, ATGP3207-1 mg, ATGP3207-10, ATGP3207-20, ATGP3207-50, ATGP3207-100, ATGP3207-250, ATGP3207-500, ATGP3207-1

Yang N., et al. (2007) Mol Endocrinol. 21:1642-1655.; ; Ross RJ., et al. (1997) Mol Endocrinol. 11:265-273.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_000154

P10912

Human