Recombinant humaan GRP58/PDIA3-eiwit

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

Protein disulfide isomerase family A member 3, Glucose regulated protein 58kDa, GPR58, Protein disulfide isomerase-associated 3, 58 kDa microsomal protein, P58, ERp61, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, GRP57, PI-PLC, HsT17083

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity > 10 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.

58.5 kDa (518aa) confirmed by MALDI-TOF

ERp57, PDIA3, Protein disulfide-isomerase A3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, ATGP3137-10 µg, ATGP3137-20 µg, ATGP3137-50 µg, ATGP3137-100 µg, ATGP3137-250 µg, ATGP3137-500 µg, ATGP3137-1 mg, ATGP3137-10, ATGP3137-20, ATGP3137-50, ATGP3137-100, ATGP3137-250, ATGP3137-500, ATGP3137-1

Vigneron N., et al. (2009) Eur J Immunol. 39 (9) :2371-6. ; ; Forster ML., et al. (2009) J Biol Chem. 284 (19) :13045-56.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,0.1 M NaCl, and 10% glycerol

Heat Shock Proteins

NP_005304

P30101

Human

MSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL