Recombinant humaan GRP78/HSPA5-eiwit

Binding immunoglobulin protein (BiP) belongs to the family of ~70 kDa heat shock proteins (HSP 70) . It is a stress response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding as well as for the maintenance of cell homeostasis and the prevention of apoptosis. Recombinant human BIP protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Heat shock protein family A member 5, Heat shock 70kD protein 5, HSP70 family protein 5, Glucose-regulated protein 78kD, Binding-immunoglobulin protein, BiP, Endoplasmic reticulum chaperone BiP, Glucose-regulated protein 78kDa, Immunoglobulin heavy chain-binding protein

Baculovirus

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

71 kDa (640aa)

GRP78, HSPA5, Binding immunoglobulin protein, HSPA5, heat shock 70kDa protein 5, GRP78, glucose-regulated protein 78kD, MIF2, ATGP0824-10 µg, ATGP0824-20 µg, ATGP0824-50 µg, ATGP0824-100 µg, ATGP0824-250 µg, ATGP0824-500 µg, ATGP0824-1 mg, ATGP0824-10, ATGP0824-20, ATGP0824-50, ATGP0824-100, ATGP0824-250, ATGP0824-500, ATGP0824-1

Lee LC., et al. (2009) Clin Chim Acta. 400 (1-2) :56-62. ; ; Racek T., et al. (2008) J Biol Chem. 283 (49) :34305-14.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 200 mM NaCl

Heat Shock Proteins

NP_005338.1

P11021

Human