Recombinant humaan GRPEL1-eiwit

GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Recombinant human GRPEL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GrpE protein homolog 1 mitochondrial, FLJ25609, HMGE

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.6 kDa (211aa) confirmed by MALDI-TOF

GRPEL1, grpE protein homolog 1 mitochondrial, FLJ25609, HMGE, ATGP0745-10 µg, ATGP0745-20 µg, ATGP0745-50 µg, ATGP0745-100 µg, ATGP0745-250 µg, ATGP0745-500 µg, ATGP0745-1 mg, ATGP0745-10, ATGP0745-20, ATGP0745-50, ATGP0745-100, ATGP0745-250, ATGP0745-500, ATGP0745-1

Borges JC., et al. (2003) J Biol Chem. 278 (37) :35337-44.; ; Hohfeld J., et al. (1997) EMBO J. 16 (20) :6209-16.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Heat Shock Proteins

NP_079472

Q9HAV7

Human

MGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV EEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK EPGTVALVSK VGYKLHGRTL RPALVGVVKE A