Recombinant humaan GSK-3 bèta-eiwit

GSK3B, also known as glycogen synthase kinase-3 beta, acts as a negative regulator in the hormonal control of glucose homeostasis. Also, this protein has diverse functions as Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Recombinant human GSK3B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

GSK-3 beta, Serine/threonine-protein kinase GSK3B

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

47.5 kDa (426aa)

GSK3 b, GSK-3 beta, Serine/threonine-protein kinase GSK3B, ATGP3221-10 µg, ATGP3221-20 µg, ATGP3221-50 µg, ATGP3221-100 µg, ATGP3221-250 µg, ATGP3221-500 µg, ATGP3221-1 mg, ATGP3221-10, ATGP3221-20, ATGP3221-50, ATGP3221-100, ATGP3221-250, ATGP3221-500, ATGP3221-1

Desai SS., et al. (2014) J. Biol. Chem. 289 (9) :5386-5398.; ; Cheng DD., et al. (2014) Biochem. Biophys. Res. Commun. 443 (2) :598-603.

Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016) {https://pubmed.ncbi.nlm.nih.gov/27040940/}; ; Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017) {https://pubmed.ncbi.nlm.nih.gov/28536714/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 0.5mM PMSFand 30% glycerol

Neuroscience

NP_001139628.1

P49841

Human