Recombinant humaan GSKIP-eiwit

GSKIP (GSK3 beta interaction protein) is defined as a naturally occurring protein that is homologous with the GSK3beta interaction domain of Axin and is able to negatively regulate GSK3beta of the Wnt signaling pathway. GSKIP may affect GSK3beta activity on neurite outgrowth by inhibiting the specific phosphorylation of tau (ser396) . Recombinant human GSKIP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

GSK3-beta interaction protein

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.8 kDa (159aa) confirmed by MALDI-TOF

GSKIP, GSK3-beta interaction protein, ATGP1007-10 µg, ATGP1007-20 µg, ATGP1007-50 µg, ATGP1007-100 µg, ATGP1007-250 µg, ATGP1007-500 µg, ATGP1007-1 mg, ATGP1007-10, ATGP1007-20, ATGP1007-50, ATGP1007-100, ATGP1007-250, ATGP1007-500, ATGP1007-1

Lin CC, et al. (2009) J Cell Biochem. 108 (6) :1325-36.; ; Chou HY, et al (2006) Biochemistry. 45 (38) :11379-89.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Metabolism

NP_057556

Q9P0R6

Human

MGSSHHHHHH SSGLVPRGSH METDCNPMEL SSMSGFEEGS ELNGFEGTDM KDMRLEAEAV VNDVLFAVNN MFVSKSLRCA DDVAYINVET KERNRYCLEL TEAGLKVVGY AFDQVDDHLQ TPYHETVYSL LDTLSPAYRE AFGNALLQRL EALKRDGQS